Spoliogotyping - A key to Genetic insights
Spoligotyping Spoligotyping is a molecular biological method used to describe the genetic diversity of Mycobacterium tuberculosis complex (MTBC) species in humans and animals. It involves amplifying a specific DNA region called the direct repeat (DR) region using oligonucleotide primers and then hybridizing the PCR product to a set of immobilized oligonucleotides corresponding to unique spacer DNA sequences within the DR locus. The results are interpreted by converting black squares to 1 and white squares to 0, which are then transferred to databases for further analysis. Spoligotyping helps identify spoligotype patterns, lineages, and international types of MTBC isolates, providing valuable information for understanding TB epidemiology and transmission dynamic. Fig. 1: Genetic characterization of Mycobacterium tuberculosis strains from different region...